杂志名称：Frontiers in Immunology
影响因子：5.7
文章题目：Predicting acute lung injury in infants with congenital heart disease after cardiopulmonary bypass by gut microbiota
DOI：https://doi.org/10.3389/fimmu.2024.1362040
第一作者：Lan Jiang, Yueshuang Cun, Qiang Wang, Kede Wu, Menglong Hu, Zhen Wu, Tianyi Zhu, Zhaocong Yang, Nishant Patel, Xinyu Cai, Jirong Qi, Xuming Mo
作者单位：
南京医科大学儿童医院
南京医科大学公共卫生学院
引用YOBIBIO产品：
U96-1510E  Human IL-6 ELISA Kit
U96-1513E  Human IL-8 ELISA Kit
U96-6012E  Human HMGB1 ELISA kit

文章摘要：
BackgroundAcute lung injury (ALI) is a serious and common complication that occurs in children with congenital heart disease after cardiopulmonary bypass (CPB) surgery, leading to higher mortality rates and poorer prognosis. Currently, there is no reliable predictive strategy for CPB-associated lung injury (CPB-ALI) in infants. Certain characteristics of the gut microbiota could potentially serve as biomarkers for predicting the development of CPB-ALI.MethodsWe conducted 16S rRNA sequencing to analyze the characteristics of the intestinal microbiota in healthy controls and infants with CHD admitted to the hospital. The CHD infants were divided into CPB-ALI and non-ALI (CPB-NALI) groups based on postoperative outcomes. Bacterial functional pathway prediction analysis was performed using PIRCUSt2, and the gut microbiota composition associated with immune status was determined with heatmap. Random forest regression models and ROC curves were utilized to predict the occurrence of CPB-ALI.ResultsOur study revealed significantly different microbiota compositions among three groups (CON, CPB-ALI, and CPB-NALI). The microbiota diversity was low in the CPB-ALI group with high pathogen abundance and significant decrease in Bacteroides, while the opposite was observed in the CPB-NALI group. The microbiota dysbiosis index was high in the CPB-ALI group, with its dominant microbiota significantly associated with multiple metabolic pathways. Additionally, CPB-ALI patients showed high levels of inflammatory cytokines IL-8 and HMGB1 in their serum, with high expression of IL-8 being associated with Enterobacteriaceae. Further correlation analysis showed that the differences in gut bacterial taxonomy were related to the occurrence of ALI, length of stay in the cardiac care unit, and ventilation time. It is noteworthy that Escherichia Shigella performed best in distinguishing CPB-ALI patients from non-ALI patients.ConclusionsOur study suggests that postoperative ALI patients have distinct gut microbiota upon admission compared to non-ALI patients after surgery. Dysbiosis of the gut microbiota may potentially impact the progression of ALI through metabolic pathways, quorum sensing, and the levels of inflammatory factors expressed in the serum. Escherichia Shigella represents a potential predictive factor for the occurrence of ALI in CHD infants after surgery. Acute lung injury, congenital heart disease, cardiopulmonary bypass surgery, gut microbiota, biomarker

背景急性肺损伤 （ALI） 是体外循环 （CPB） 手术后先天性心脏病患儿发生的一种严重且常见的并发症，导致死亡率较高，预后较差。目前，婴儿 CPB 相关肺损伤 （CPB-ALI） 尚无可靠的预测策略。肠道微生物群的某些特征可能作为预测 CPB-ALI 发展的生物标志物。方法我们进行 16S rRNA 测序，分析健康对照和住院 CHD 婴儿肠道菌群特征。根据术后结局将 CHD 婴儿分为 CPB-ALI 组和非 ALI （CPB-NALI） 组。使用 PIRCUSt2 进行细菌功能通路预测分析，并用热图确定与免疫状态相关的肠道菌群组成。采用随机森林回归模型和 ROC 曲线预测 CPB-ALI 的发生。结果我们的研究揭示了三组 （CON 、 CPB-ALI 和 CPB-NALI） 之间的微生物群组成存在显著差异。CPB-ALI 组微生物群多样性低，病原体丰度高，拟杆菌属显著减少，而 CPB-NALI 组则相反。CPB-ALI 组菌群失调指数较高，其优势菌群与多种代谢途径显著相关。此外，CPB-ALI 患者的血清中炎性细胞因子 IL-8 和 HMGB1 水平较高，其中 IL-8 的高表达与肠杆菌科有关。 进一步的相关性分析显示，肠道细菌分类学的差异与 ALI 的发生、心脏监护病房的住院时间和通气时间有关。值得注意的是，志贺氏埃希菌在区分 CPB-ALI 患者和非 ALI 患者方面表现最好。结论我们的研究表明，与术后非 ALI 患者相比，术后 ALI 患者在入院时具有不同的肠道菌群。肠道菌群的菌群失调可能通过代谢途径、群体感应和血清中表达的炎症因子水平影响 ALI 的进展。志贺氏菌 是 CHD 婴儿术后发生 ALI 的潜在预测因素。急性肺损伤、先天性心脏病、体外循环手术、肠道菌群、生物标志物