杂志名称：Bioactive Materials
影响因子：18.0
文章题目：Biguanide chitosan microneedles with cell-free DNA scavenging ability for psoriasis therapy
DOI：https://doi.org/10.1016/j.bioactmat.2023.11.015
第一作者：Zihao Liu, Yu Wang, Yuhan Zhang, Liufu Hu, Bozhi Chen, Yang Li, Xindong Guo, Bingran Yu, Fu-Jian Xu
作者单位：北京化工大学
引用YOBIBIO产品：
U96-1494E  Mouse IL-1β ELISA Kit

文章摘要：
High levels of cell-free DNA (cfDNA) induce psoriasis. Currently, the treatment of psoriasis has the disadvantages of penetration difficulty, suppression of normal immunity, and skin irritation. In this study, biguanide chitosan microneedles (BGC-MNs) were prepared to treat psoriasis by removing cfDNA from the dermis through the skin barrier. The effects of chitosan with different bisguanidine contents on DNA-binding capacity, biocompatibility, and inflammation inhibition were compared, revealing that chitosan containing 20% bisguanidine (BGC2) was found to have the best overall performance. BGC2 effectively cleared cfDNA and inhibited the production of inflammatory factors. BGC-MN made from BGC2 had good mechanical and solubility properties. BGC-MNs cleared cfDNA, reduced the level of inflammatory factors in the dermis, and exerted a good therapeutic effect on mice with psoriasis. These results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.

高水平的游离 DNA (cfDNA) 会诱发牛皮癣。目前银屑病的治疗存在渗透困难、正常免疫力受到抑制、皮肤刺激等缺点。在这项研究中，制备了双胍壳聚糖微针（BGC-MNs），通过皮肤屏障去除真皮中的 cfDNA 来治疗牛皮癣。比较了不同双胍含量的壳聚糖对DNA结合能力、生物相容性和炎症抑制的影响，发现含有20%双胍的壳聚糖（BGC2）具有最佳的综合性能。 BGC2有效清除cfDNA并抑制炎症因子的产生。由BGC2制成的BGC-MN具有良好的机械性能和溶解性能。 BGC-MNs可清除cfDNA，降低真皮内炎症因子水平，对银屑病小鼠发挥良好的治疗作用。这些结果表明，BGC-MNs 在清除 cfDNA 和发挥抗炎作用方面为治疗银屑病提供了一种新方法。