杂志名称：Advanced Science
影响因子：14.3
文章题目：An Integrated Whole‐Process Repair System with Programmed Regulation of Healing Performance Facilitates Urethral Wound Restoration and Scarless Reconstruction
DOI: https://doi.org/10.1002/advs.202409930
第一作者：Wenzhuo Fang, Ying Wang, Kaile Zhang, Ming Yang, Meng Liu, Yangwang Jin, Xianjie Xiu, Yuhui Wang, Zhenwei Yu, Ranxing Yang, Qiang Fu
作者单位：上海交通大学医学院附属第六人民医院
引用YOBIBIO产品：
U96-1640E  Rat VEGF/VEGFA ELISA Kit
U96-2901E  Rat PDGF-BB ELISA Kit
U96-2054E  Rat EGF ELISA Kit
U96-1738E  Rat bFGF/FGF2 ELISA Kit
U96-2401E  Rat HGF ELISA Kit

文章摘要：
The harsh microenvironment of the urethral injury often carries high risks of early undesirable healing as well as late scar tissue formation. Indeed, the infection and inflammatory response in the early stages as well as blood vessel formation and tissue regeneration in the later stages fundamentally impact the outcomes of urethral wound healing. Innovatively, an integrated whole‐process repair hydrogel (APF/C/L@dECM) is designed. After rigorous testing, it is found that hydrogels formed by hydrophobic association and double cross‐linking of amide bonds can procedurally regulate wound healing in all phases to match the repair process. In rabbit models of urethral wound, APF/C/L@dECM hydrogel can achieve scarless reconstruction with significantly better results than other hydrogels. Noteworthily, multi‐stage mechanistic explorations reveal the expression profiles of inflammation, vascularization, and extracellular matrix secretion‐related genes in wound tissue at different times. In summary, this study develops an overall treatment for urethral injury through a whole‐process repair system that promotes healthy healing of urethral wounds and prevents the formation of scar tissue.

尿道损伤的恶劣微环境通常具有早期不良愈合和晚期疤痕组织形成的高风险。事实上，早期的感染和炎症反应以及后期的血管形成和组织再生从根本上影响尿道伤口愈合的结果。创新性地设计了一种一体化全过程修复水凝胶 （APF/C/L@dECM）。经过严格测试，发现由疏水缔合和酰胺键双交联形成的水凝胶可以在所有阶段程序上调节伤口愈合以匹配修复过程。在尿道伤口的兔模型中，APF/C/L@dECM 水凝胶可以实现无疤痕重建，效果明显优于其他水凝胶。值得注意的是，多阶段机制探索揭示了伤口组织中炎症、血管形成和细胞外基质分泌相关基因在不同时间的表达谱。综上所述，本研究通过全程修复系统开发了一种整体治疗尿道损伤的方法，该系统可以促进尿道伤口的健康愈合并防止疤痕组织的形成。