PRODUCT NAME: Goat Anti-Rabbit IgG (FITC)

CATALOG NUMBER: YB7025

HOST: Goat

ISOTYPE: Goat IgG

REACTIVITY: Rabbit

APPLICATIONS: IHC, ICC/IF, FC

ALL NAMES: Fluorescein (FITC)；FITC

PURITY: Affinity-chromatography

STORAGE/STABILITY: Store at +4℃ short term. Store at -20℃ for long term. Avoid freeze / thaw cycle.

BUFFER: Goat IgG in 10mM phosphate buffered saline, pH7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

APPLICATION NOTES: ICC/IF 1:100-1:500    IHC 1:100-1:500   FC：1:100-1:500

STORAGE/STABILITY: Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.

BACKGROUND: FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492nm and fluoresce maximally at 520nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor 488 because it is brighter and more photostable.

Immunofluorescent analysis of Hela cells.