Anti-GAPDH-Peroxidase (HRP) Conjugated

PRODUCT NAME：Anti-GAPDH-Peroxidase (HRP) Conjugated

CATALOG NUMBER：U33-841A-S

LOT NUMBER：U33022

ISOTYPE：Mouse IgG1

Clone NO：1B20

SOURCE：Mouse Ascites

PURIFICATION：Protein A purified

QUANTITY：100 μl

DESCRIPTION：Monoclonal antibody is produced by immunizing mouse with synthetic peptide [CRLEKPAKYDDIKK], coupled to KLH.

REACTIVITY：human, mouse, African green monkey

APPLICATIONS：Western Blot

BUFFER：10mM sodium phosphate, 150mM sodium chloride, pH 7.4 with 50% glycerol

PROTEIN CONCENTRATION：approximately 1 mg/ml

RECOMMMENED ANTIBODY DILUTION：Western blot: 1:4000

STORAGE/STABILITY：Antibody-HRP is recommended being stored at -20℃.

Background：

The enzyme glyceraldehydes-3-phosphate dehydrogenase is a tetramer of identical chains that catalyzes the reversible oxidative phosphorylation of glyceraldehydes-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide. This is an important energy-yielding step in carbohydrate metabolism. GAPDH is found in almost all species with low rate of evolutionary changes.