Monoclonal Anti-β-actin-Peroxidase (HRP) Conjugated

PRODUCT NAME：Monoclonal Anti-β-actin-Peroxidase (HRP) Conjugated      

CATALOG NUMBER：U33-486A-S

LOT NUMBER：U33022

ISOTYPE：Mouse IgG1

Clone NO：1B20

SOURCE：Mouse Ascites

QUANTITY：100μl

DESCRIPTION：Monoclonal antibody is produced by immunizing mouse with synthetic peptide [D(Ac)DDIAALVIDNGSGLC], coupled to KLH.

REACTIVITY：human, mouse, African green monkey

APPLICATION：Western Blot

BUFFER：10mM sodium phosphate, 150mM sodium chloride, pH 7.4 with 50% glycerol

PROTEIN CONCENTRATION：approximately 1mg/ml

RECOMMENDED ANTIBODY DILUTION：Western Blot: 1:4000

STORAGE/STABILITY：Antibody-HRP is recommended being stored at -20℃.

Background:

Actin is one of the most conserved eukaryotic proteins. It is expressed in mammals and birds with at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Our monoclonal anti-β-actin-peroxidase (HRP) conjugated provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.