Anti-Flag agrose beads/Anti-Flag Affinity Gel
PRODUCT NAME:Anti-Flag agrose beads/Anti-Flag Affinity Gel
CATALOG NUMBER: U33-135A-F
LOT NUMBER:U33022
ISOTYPE:IgG2b
Clone NO:1B20
QUANTITY:5ml
DESCRIPTION:Anti-Flag Affinity Gel is a purified murine IgG2b Monoclonal antibody covalently attached to sepharose 4B
APPLICATION:rProtein Purification, Immunoprecipitation
REAGENT:Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.
ANTIBODY BOUND:NLT 7.5 grams antibody/ Liter gel
RECOMMENDED VOLUME:IP: 5μl gel for 500μl crude protein solution
BINDING CAPACITY:Minimum 1.1mg of a Flag fusion protein eluted per ml of packed resin
SETTING VOLUME:10 ml resin slurry
STORAGE/STABILITY:
Anti-Flag Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol