Monoclonal Anti-His Tag-Peroxidase (HRP) Conjugated
PRODUCT NAME:Monoclonal Anti-His Tag-Peroxidase (HRP) Conjugated
CATALOG NUMBER:U33-421A-S
LOT NUMBER:U33022
ISOTYP:Mouse IgG1
Clone NO:1B20
SOURCE: Mouse Ascites
PURIFICATION:Protein A purified
QUANTITY:400μl
DESCRIPTION:Monoclonal antibodies are produced by immunizing mouse with synthesized polypeptide 6 x His, coupled with KLH. This antibody can recognize the His-tag (HHHHHH) fused to either the amino or carboxy terminal of targeted protein.
APPLICATION:Western Blot
REAGENT:1×PBS with 50% glycerol
PROTEIN CONCENTRATION:approximately 1mg/ml
RECOMMENDED ANTIBODY DILUTION:Western Blot: 1:4000
STORAGE/STABILITY:Antibody-HRP is recommended being stored at -20℃.
Background:
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibody have been widely used in study of protein expression in various systems. Our anti-His Tag-peroxidase conjugated monoclonal antibody provides a simple solution to detect the expression of protein with a His tag.