Anti-Strep II Monoclonal Antibody

PRODUCT NAME：Anti-Strep II Monoclonal Antibody

CATALOG NUMBER： U33-316A

LOT NUMBER：U33022

ISOTYPE：Mouse IgG1 

Clone NO：1B20

SOURCE：Mouse Ascites

QUANTITY：100μl

DESCRIPTION：Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (WSHPQFEK) coupled to KLH.

APPLICATION：Western Blot, Immunoprecipitation

REAGENT：1×PBS with 50% glycerol

PROTEIN CONCENTRATION：0.5 mg/ml

RECOMMENDED ANTIBODY DILUTION：Western Blot: 1μg/ml

STORAGE/STABILITY：Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.

Background：

Streptavidin is a tetrameric protein expressed in Streptomyces avidinii. Because of its high affinity for the vitamin h-biotin, Streptavidin is commonly used in the fields of molecular biology and biotechnology. The Strep-tag was originally selected from a genetic library to specifically bind to a proteolytically truncated "core" version of streptavidin. Over the years, the Strep-tag was systemically optimized, to permit a greater flexibility in the choice of attachment site. Further, its interaction partner, Streptavidin was also optimized to increase peptide-binding capacity, which resulted in the development of Strep-Tactin. The so called Strep-tag system, consisting of Strep-tag and Strep-Tactin, has proven particularly useful for the functional isolation and analysis of protein complexes in proteome research.